Freeze-fracture-autoradiography

A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-m...

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Bibliographic Details
Published in:Histochemistry 1976, Vol.50 (2), p.91-101
Main Authors: Rix, E, Schiller, A, Taugner, R
Format: Article
Language:English
Subjects:
Animals
Autoradiography - methods
Diagnostic Errors
Female
Freeze Fracturing
Ice
Male
Rana esculenta
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Summary:A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.
ISSN:0301-5564
1432-119X
DOI:10.1007/bf00495820