Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type

The distribution of a bombesin-like immunoreactive peptide in the avian gastro-intestinal tract was analysed by combined radioimmunoassay and immunocytochemistry. Radioimmunoassay of tissue extracts showed that the largest quantities of bombesin-like immunoreactivity were present in the proventricul...

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Bibliographic Details
Published in:Histochemistry 1979-01, Vol.61 (2), p.213-221
Main Authors: Timson, C M, Polak, J M, Wharton, J, Ghatei, M A, Bloom, S R, Usellini, L, Capella, C, Solcia, E, Brown, M R, Pearse, A G
Format: Article
Language:English
Subjects:
Animals
Bombesin
Chickens
Fluorescent Antibody Technique
Intestines - analysis
Intestines - ultrastructure
Microscopy, Electron
Peptides - analysis
Radioimmunoassay - methods
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Summary:The distribution of a bombesin-like immunoreactive peptide in the avian gastro-intestinal tract was analysed by combined radioimmunoassay and immunocytochemistry. Radioimmunoassay of tissue extracts showed that the largest quantities of bombesin-like immunoreactivity were present in the proventriculus (64.5 +/- 6.0 pmol/g) with smaller but still considerable amounts in the gizzard (40.0 +/- 6.0 pmol/g). Immunocytochemically the extractable bombesin-like immunoreactivity was localised in numerous endocrine cells. These, in the proventriculus, were found mainly in the deeper layers of the mucosa. Further study of these cells by the semi-thin/thin technique revealed the presence of characteristic secretory granules. The functional name BN is proposed for this cell type.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00496533