Quantitative immunohistochemistry of metallothionein in rat placenta

The presence of metallothionein (MT) was demonstrated in placentae from cadmium-exposed and control rats by an immunohistochemical technique, using peroxidase as label and the diaminobenzidine procedure for the staining reaction. The protein was found in different regions of the placenta, i.e. in tr...

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Bibliographic Details
Published in:Histochemistry 1989-01, Vol.90 (5), p.365-369
Main Authors: HAZELHOFF ROELFZEMA, W, TOHYAMA, C, NISHIMURA, H, NISHIMURA, N, MORSELT, A. F. W
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cadmium - pharmacology
Densitometry - methods
Female
Fundamental and applied biological sciences. Psychology
Immunohistochemistry - methods
Metalloproteins
Metallothionein - analysis
Other metalloproteins
Placenta - analysis
Pregnancy
Proteins
Rats
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Summary:The presence of metallothionein (MT) was demonstrated in placentae from cadmium-exposed and control rats by an immunohistochemical technique, using peroxidase as label and the diaminobenzidine procedure for the staining reaction. The protein was found in different regions of the placenta, i.e. in trophoblastic labyrinth, in spongiotrophoblast and in visceral yolk sac. Cytophotometric analysis of the final reaction product revealed that the amount of MT was increased in the placental labyrinth of cadmium-exposed rats. Increases were found in both nuclei and cytoplasm of trophoblast cells in the labyrinth region. Possible roles of MT in the transport of zinc and in the carbohydrate metabolism are discussed.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00508314