Histochemistry of carboxyl esterases in the broad bean root tip with indoxyl substrates

The possibility of the use of indoxyl substrates for enzyme localization in plant material was tested and proved. Studying the localization of the carboxyl esterase in broad ben root tip a) the time course of the indigogenic reaction was investigated, b) the concentration of the ferro/ferri redox bu...

Full description

Saved in:
Bibliographic Details
Published in:Histochemistry 1977-03, Vol.53 (1), p.79-87
Main Author: Benes
Format: Article
Language:English
Subjects:
Carboxylic Ester Hydrolases - analysis
Histocytochemistry
Indoles
Plants - enzymology
Staining and Labeling
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The possibility of the use of indoxyl substrates for enzyme localization in plant material was tested and proved. Studying the localization of the carboxyl esterase in broad ben root tip a) the time course of the indigogenic reaction was investigated, b) the concentration of the ferro/ferri redox buffer was examined, c) various substances were used to control the indigogenic visualizing reaction, d) the azocoupling procedure was applied with indoxyl substrates, e) unhalogenated and halogenated substrates were tried, and, finally, f) acetate and butyrate were employed in the indigogenic procedure. Using the last mentioned (f) trial the existence and localization was verified in the material tested of carboxyl esterase fractions recognized there previously on the basis of azocoupling reactions with naphtholic substrates.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00511212