Proliferation pattern of hamster melanoma cells cultured in diffusion chambers in pre-immunized hosts

Fortner M Mel 1 melanoma cells from the Golden Hamster were capable of exponential proliferation during intraperitoneal diffusion chamber culture in xenogenic host animals (Balb/c mice, BD IX and Marshall rats). When the host animals had been preimmunized with these melanoma cells, rapid cell lysis...

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Bibliographic Details
Published in:Archiv für dermatologische Forschung 1975, Vol.251 (3), p.169-180
Main Authors: Schieferstein, G, Laerum, O D
Format: Article
Language:English
Subjects:
Animals
Cell Count
Cell Division
Cell Line
Cricetinae
Cytotoxicity Tests, Immunologic
Diffusion
Female
Immunization
Melanoma - immunology
Melanoma - pathology
Mice
Neoplasm Transplantation
Peritoneal Cavity
Rats
Skin Neoplasms - immunology
Skin Neoplasms - pathology
Transplantation Immunology
Transplantation, Heterologous
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Summary:Fortner M Mel 1 melanoma cells from the Golden Hamster were capable of exponential proliferation during intraperitoneal diffusion chamber culture in xenogenic host animals (Balb/c mice, BD IX and Marshall rats). When the host animals had been preimmunized with these melanoma cells, rapid cell lysis was observed in the chambers within 4 hrs after implantation. The cell numbers were reduced to about 1 - 10% of the inoculum. At this level the cell numbers persisted for several days, as opposed to control cultures in untreated hosts, where the cells proliferated rapidly. After 6 - 10 days the cell numbers in chambers from immunized hosts slowly increased in spite of a high level of cytotoxicity in ther serum of the animals. The same pattern was seen after previous immunization with hamster lymphocytes, indicating that the antibodies were directed against a common antigen for melanoma cells and lymphocytes in hamsters. The diffusion chamber method appears to be a useful system for the study of a pure humoral immune reaction against tumour cells, and especially for investigation of the phenomenon that some tumour cells are able to escape a strong humoral cytotoxic effect from host animals.
ISSN:0003-9187
0340-3696
1432-069X
DOI:10.1007/BF00561758