A rapid method for the preparation of the neutrophil fraction of granulocytes from human blood by centrifugation on isotonic nycodenz gradients

A rapid method for the preparation of neutrophils of the granulocyte fraction of human blood is described. A leucocyte-rich fraction is loaded onto preformed isotonic Nycodenz gradients, formed by mixing a stock isotonic Nycodenz solution with a NaCl diluent solution. After low speed centrifugation...

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Bibliographic Details
Published in:Blut 1987-06, Vol.54 (6), p.337-342
Main Authors: FORD, T. C, NEEDLE, R, RICKWOOD, D
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Separation - methods
Cell Survival
Centrifugation, Density Gradient - methods
Diverse techniques
Fundamental and applied biological sciences. Psychology
Granulocytes - cytology
Humans
Iohexol
Molecular and cellular biology
Neutrophils - cytology
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Summary:A rapid method for the preparation of neutrophils of the granulocyte fraction of human blood is described. A leucocyte-rich fraction is loaded onto preformed isotonic Nycodenz gradients, formed by mixing a stock isotonic Nycodenz solution with a NaCl diluent solution. After low speed centrifugation a neutrophil fraction of high viability, yield and purity can be isolated from the gradients.
ISSN:0006-5242
1432-0584
DOI:10.1007/BF00626014