Quantitative distribution of lysosomal hydrolases in the rat nephron

The activities of N-acetyl-beta, D-glucosaminidase (NAG, EC 3.2.1.30), beta, D-galactosidase (beta-gal, EC 3.2.1.23) and acid phosphatase (ac-Pase, EC 3.1.3.2) were measured in the glomeruli, five segments of the proximal and four segments of the distal tubule of normal male Wistar rats. The activit...

Full description

Saved in:
Bibliographic Details
Published in:Histochemistry 1979-01, Vol.63 (2), p.245-251
Main Authors: Le Hir, M, Dubach, U C, Schmidt, U
Format: Article
Language:English
Subjects:
Acetylglucosaminidase - metabolism
Acid Phosphatase - metabolism
Animals
Galactosidases - metabolism
Hydrolases - metabolism
Lysosomes - enzymology
Male
Nephrons - enzymology
Rats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The activities of N-acetyl-beta, D-glucosaminidase (NAG, EC 3.2.1.30), beta, D-galactosidase (beta-gal, EC 3.2.1.23) and acid phosphatase (ac-Pase, EC 3.1.3.2) were measured in the glomeruli, five segments of the proximal and four segments of the distal tubule of normal male Wistar rats. The activities of NAG and beta-gal are 3- to 5-fold higher in the first part of the proximal tubule than in other segments and very low in glomeruli. We propose that the distribution of these two glycosidases reflects the contribution of the different tubular segments to the reabsorption of glycoproteins. The maximal activity of ac-Pase was found in the straight part of the proximal tubule. It was only 1.5-fold higher than in the distal tubule. Moreover, the activity in glomeruli is rather high. We conclude that ac-Pase is not primarily involved in the handling of reabsorbed molecules.
ISSN:0301-5564
1432-119X
DOI:10.1007/bf00644546