Calcifying odontogenic cyst immunohistochemical detection of keratin and involucrin in cyst wall

Calcifying odontogenic cysts (COC) were immunohistochemically described using different keratin proteins and involucrin as well as histopathology. The cystic lining epithelium was composed of calcifying, keratinizing, squamous, and columnar epithelial cells, and included calcified masses of irregula...

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Bibliographic Details
Published in:Virchows Archiv A Pathological Anatomy and Histopathology 1988-01, Vol.412 (3), p.189-196
Main Authors: YAMAMOTO, Y, HIRANUMA, Y, EBA, M, OKITSU, M, UTSUMI, N, TAJIMA, Y, TATEMOTO, Y, MORI, M
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Child
Epithelial Cells
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
Gingival Neoplasms - pathology
Humans
Immunohistochemistry
Keratins - analysis
Keratins - immunology
Male
Medical sciences
Odontogenic Tumors - pathology
Otorhinolaryngology. Stomatology
Protein Precursors - analysis
Protein Precursors - immunology
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Summary:Calcifying odontogenic cysts (COC) were immunohistochemically described using different keratin proteins and involucrin as well as histopathology. The cystic lining epithelium was composed of calcifying, keratinizing, squamous, and columnar epithelial cells, and included calcified masses of irregular shape and various size as well as ghost cells. Calcifying epithelium gave negative or only trace staining for keratins detected with low molecular keratin (PKK1), but were regularly positive with high molecular keratin (KL1) and polyclonal antibody for keratin (TK). They were occasionally positive for involucrin. The cells located in the periphery of the calcified masses had a particular abundance of high molecular weight and total keratins (KL1 and TK). Calcified bodies and ghost cells were devoid of any immunoreactivity. Squamous epithelium was relatively similar to that of normal squamous cell epithelium in the oral mucosa. It were most commonly found in columnar cystic epithelial cells which displayed intense staining with all immunoreagents. It is postulated that such epithelial cells may have a strong potentiality to transform into ghost cells or to undergo metaplasia. They may develop altered synthesis of homogenous acellular materials and finally become transformed into calcifying epithelium containing dystrophic calcified masses.
ISSN:0174-7398
1432-2307
DOI:10.1007/BF00737142