Echinococcus multilocularis: in vitro interactions between protoscolices and Kupffer cells

Echinococcus multilocularis protoscolices collected from experimentally infected jirds were incubated for 2 weeks in rat hepatic cell cultures cocultivated with or without feeder cells (BALB/c 3T3 and IAR 20). Scanning and transmission electron microscopy studies were performed during the course of...

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Bibliographic Details
Published in:Parasitology research (1987) 1994-05, Vol.80 (5), p.381-387
Main Authors: WALBAUM, S, AL NAHHAS, S, GABRION, C, MESNIL, M, PETAVY, A. F
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Adhesion
Cells, Cultured
Echinococcus - growth & development
Echinococcus - ultrastructure
Gerbillinae
Glycoproteins
Helminthic diseases
Infectious diseases
Kupffer Cells - cytology
Kupffer Cells - parasitology
Kupffer Cells - ultrastructure
Liver - cytology
Liver - parasitology
Liver - ultrastructure
Medical sciences
Mice
Mice, Inbred Strains
Microscopy, Electron
Miscellaneous
Parasitic diseases
Phagocytosis
Polysaccharides
Rats
Rats, Inbred Strains
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Summary:Echinococcus multilocularis protoscolices collected from experimentally infected jirds were incubated for 2 weeks in rat hepatic cell cultures cocultivated with or without feeder cells (BALB/c 3T3 and IAR 20). Scanning and transmission electron microscopy studies were performed during the course of the culture period. Kupffer cells (Kc) were seen adhering to the anterior and posterior ends of the protoscolices. Some protoscolices were fixed to the cell monolayer by a cluster of Kc adhering to the posterior end of the parasite. These cells were phagocytosing the glycocalyx and the electron-dense distal end of the microthrix of the protoscolex tegument. An alteration in the superficial tegumental cytoplasm with extensive mitochondrial damage was also noted. The properties expressed by Kc against protoscolices in vitro might also be relevant for proliferation of metacestodes in vivo.
ISSN:0932-0113
1432-1955
DOI:10.1007/BF00932375