Erythroid colony formation (CFUe) in fetal liver and adult bone marrow and spleen from the mouse

The methyl cellulose modification of the CFUe technique has been applied to 14 day fetal liver and adult bone marrow and spleen from CBA/CA mice. Optimized doses of fetal calf serum, alpha-thioglycerol, erythropoietin and cell suspensions have been obtained from dose response curves in order to stan...

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Bibliographic Details
Published in:Blut 1976-09, Vol.33 (3), p.171-180
Main Authors: Rich, I N, Kubanek, B
Format: Article
Language:English
Subjects:
Animals
Bone Marrow - drug effects
Bone Marrow Cells
Cattle
Cell Division - drug effects
Dose-Response Relationship, Drug
Erythropoiesis - drug effects
Erythropoietin - pharmacology
Female
Glycerol - analogs & derivatives
Glycerol - pharmacology
Hydroxyurea - pharmacology
Liver - enzymology
Methylcellulose - pharmacology
Mice
Mice, Inbred CBA
Pregnancy
Spleen - drug effects
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Summary:The methyl cellulose modification of the CFUe technique has been applied to 14 day fetal liver and adult bone marrow and spleen from CBA/CA mice. Optimized doses of fetal calf serum, alpha-thioglycerol, erythropoietin and cell suspensions have been obtained from dose response curves in order to standardize the technique. The slopes of the erythropoietin and cell dose response curves indicate a greater sensitivity by fetal liver to the hormone than bone marrow or spleen. The proportion of cells in the DNA synthesis phase of the cell cycle, as measured by the CFUe technique, has been estimated by administering hydroxyurea. Two hours after the drug was injected, 89% of fetal liver cells, 71% of bone marrow cells and 81% of spleen cells were found to be in the S-phase.
ISSN:0006-5242
1432-0584
DOI:10.1007/BF00995998