The interaction of a Huntington disease factor with receptors for the neurotoxin kainic acid

A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-p...

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Bibliographic Details
Published in:Metabolic brain disease 1991-12, Vol.6 (4), p.213-224
Main Authors: DODD, P. R, WATSON, W. E. J, MORRISON, M. M, JOHNSTON, G. A. R, IAN SMITH, A, RUWOLDT, A, WALLS, R. S
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - metabolism
Cerebral Cortex - metabolism
Chromatography, High Pressure Liquid
Corpus Striatum - metabolism
Humans
Huntington Disease - metabolism
In Vitro Techniques
Kainic Acid - metabolism
Leukocytes - metabolism
Medical sciences
Nervous system involvement in other diseases. Miscellaneous
Neurology
Rats
Receptors, Kainic Acid
Receptors, Neurotransmitter - metabolism
Spectrophotometry, Ultraviolet
Subcellular Fractions - metabolism
Synaptic Membranes - metabolism
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Summary:A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [3H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid.
ISSN:0885-7490
1573-7365
DOI:10.1007/BF00996921