Cryopreservation and transfer of baboon embryos

The feasibility of modifying bovine cryopreservation methods for use with baboon embryos was evaluated. Twenty-six baboon embryos at the eight-cell to blastocyst stage of development were transferred after being frozen using glycerol as cryoprotectant. Either a two-step fast of a controlled linear t...

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Bibliographic Details
Published in:Journal of In Vitro Fertilization and Embryo Transfer 1986-02, Vol.3 (1), p.33-39
Main Authors: Pope, C E, Pope, V Z, Beck, L R
Format: Article
Language:English
Subjects:
Animals
Cattle
Cryoprotective Agents - pharmacology
Embryo Transfer
Female
Freezing
Papio
Pregnancy
Sucrose - pharmacology
Tissue Preservation - methods
Tissue Survival
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Summary:The feasibility of modifying bovine cryopreservation methods for use with baboon embryos was evaluated. Twenty-six baboon embryos at the eight-cell to blastocyst stage of development were transferred after being frozen using glycerol as cryoprotectant. Either a two-step fast of a controlled linear temperature depression was achieved to -40 degrees C, followed by plunging into liquid nitrogen. After thawing, embryos were rehydrated in medium containing sucrose (0.5 to 1.0 M) using various methods of glycerol dilution. Embryos were transferred nonsurgically to anesthetized recipient baboons. Two term pregnancies resulted from six embryos frozen using the controlled linear cooling method and rehydrated by dropwise dilution of the sucrose (0.8 M) medium. Later cell stages had a greater morphological integrity postthaw than did earlier developmental stages, and embryos frozen by the linear cooling-rate method had less cell damage than those frozen by the fast method.
ISSN:0740-7769
1573-7330
DOI:10.1007/BF01131378