Hydrogen peroxide induced reproductive and interphase death in two strains of L5178Y murine lymphoma differing in radiation sensitivity

L5178Y-R (LY-R) and L5178Y-S (LY-S) cells, differing in radiation sensitivity and susceptibility to the radiosensitizing effect of benzamide (Bz) were examined for susceptibility to hydrogen peroxide. Survival and chromatid aberration frequency indicated that LY-R cells were considerably more sensit...

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Bibliographic Details
Published in:Radiation and environmental biophysics 1991, Vol.30 (2), p.105-116
Main Authors: Bouzyk, E, Buraczewska, I, Rosiek, O, Sochanowicz, B, Szumiel, I
Format: Article
Language:English
Subjects:
Animals
Benzamides - pharmacology
Calcium - metabolism
Catalase - metabolism
Cell Survival - drug effects
Cell Survival - radiation effects
Chromatids - drug effects
Chromosome Aberrations
Drug Resistance
Fura-2
Gamma Rays
Hydrogen Peroxide - pharmacology
Interphase - drug effects
Interphase - radiation effects
Kinetics
Leukemia L5178
Mice
Models, Biological
Mutagens
X-Rays
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Summary:L5178Y-R (LY-R) and L5178Y-S (LY-S) cells, differing in radiation sensitivity and susceptibility to the radiosensitizing effect of benzamide (Bz) were examined for susceptibility to hydrogen peroxide. Survival and chromatid aberration frequency indicated that LY-R cells were considerably more sensitive to H2O2 than LY-S cells. So, LY strains were found to be inversely cross-sensitive to X/gamma rays and H2O2. The relative resistance to H2O2 corresponded with the previously found twofold difference in catalase activity (Jaworska et al. 1987). At higher concentrations H2O2 treatment caused interphase death, that was delayed by benzamide (Bz, 2mM), an inhibitor of poly(ADP-ribosylation), to a lesser extent in the more resistant cell subline (LY-S). From the examination of the H2O2 induced increase in the free Ca2+ concentration (with or without 2 mM Bz treatment) with the use of Fura-2 it followed, that the cells responded to the oxidative stress by Ca2+ release. The Ca2+ concentration increase was neither directly related to the killing effect of H2O2 treatment, nor did it correspond with the twofold difference in catalase activity in LY strains.
ISSN:0301-634X
1432-2099
DOI:10.1007/BF01219344