Respiration-deficient Chinese hamster cell mutants: biochemical characterization

We have previously classified 35 of our respiration-deficient mutants into seven complementation groups and one "overlapping" mutant which does not complement mutants from groups I and II. In this paper we report on the biochemical characterization of representatives of complementation gro...

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Bibliographic Details
Published in:Somatic Cell Genetics 1979-07, Vol.5 (4), p.441-451
Main Authors: Breen, G A, Scheffler, I E
Format: Article
Language:English
Subjects:
Animals
Carbon Dioxide - metabolism
Carbon Radioisotopes
Cell Line
Cricetinae
Cricetulus
Lung
Mitochondria - metabolism
Mutation
NADH, NADPH Oxidoreductases - metabolism
Oxygen Consumption
Succinate Dehydrogenase - metabolism
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Summary:We have previously classified 35 of our respiration-deficient mutants into seven complementation groups and one "overlapping" mutant which does not complement mutants from groups I and II. In this paper we report on the biochemical characterization of representatives of complementation groups I, II, VII, and the "overlapping" mutant. We show that these mutants all have a defect in complex I of the electron-transport chain. The general features of these mutants are: (1) a low rate of O2 consumption in whole cells; (2) a low rate of release of 14CO2 from [2-14C] pyruvate, [1-14C] pyruvate, and [3-14C] beta-hydroxybutyrate; (3) a low rate of release of 14CO2 from [5-14C] glutamate and [1-14C] glutamate in mutants from groups II, VII, and the "overlapping" mutant, whereas a significant amount of 14CO2 is released in mutants from group I; (4) a substantial rate of release of 14CO2 from [U-14C] asparate; (5) in isolated mitochondria, succinate and alpha-glycerol phosphate stimulate O2 consumption whereas substrates which generate NADH, such as malate, do not; and (6) there is little or no rotenone-sensitive NADH oxidase activity in isolated mitochondria.
ISSN:0098-0366
1572-9931
DOI:10.1007/BF01538879