Thermal inducible expression of xylose isomerase and its performance in a hollow fiber bioreactor

The Escherichia coli xylose isomerase (EC 5.3.1.5) has been expressed under the control of a thermal inverting promoter system (att-nutL-p-att-N block) and its performance in a hollow fiber bioreactor measured. The conversion of xylose to xylulose was inversely proportional to the flow rate and the...

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Bibliographic Details
Published in:Journal of Industrial Microbiology 1989, Vol.4 (1), p.1-5
Main Authors: WONG, D. W. S, YEE, L. N. H, BATT, C. A
Format: Article
Language:English
Subjects:
Biological and medical sciences
bioreactors
Biotechnology
conversion
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
heat
Methods. Procedures. Technologies
Vectors (cloning, transfer, expression). Insertion sequences and transposons
xylose
xylose isomerase
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Summary:The Escherichia coli xylose isomerase (EC 5.3.1.5) has been expressed under the control of a thermal inverting promoter system (att-nutL-p-att-N block) and its performance in a hollow fiber bioreactor measured. The conversion of xylose to xylulose was inversely proportional to the flow rate and the system operated up to 60 degree C. The maximum conversion efficiency observed was 19.05% at 55 degree C.
ISSN:0169-4146
1476-5535
DOI:10.1007/BF01569686