Problems and approaches in covalent attachment of peptides and proteins to inorganic surfaces for biosensor applications

Some of the fundamental problems in covalent attachment of peptides and proteins to putative biosensor surfaces are reviewed and specific approaches to these problems discussed. In addition, selected aspects of our recent work utilizing self-assembled monolayer (SAM) systems designed to react select...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Industrial Microbiology 1994-05, Vol.13 (3), p.137-143
Main Authors: ZULL, J. E, REED-MUNDELL, J, LEE, Y. W, VEZENOV, D, ZIATS, N. P, ANDERSON, J. M, SUKENIK, C. N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Molecular Sequence Data
Peptides - chemistry
Peptides - metabolism
Protein Binding
Proteins - chemistry
Proteins - metabolism
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Some of the fundamental problems in covalent attachment of peptides and proteins to putative biosensor surfaces are reviewed and specific approaches to these problems discussed. In addition, selected aspects of our recent work utilizing self-assembled monolayer (SAM) systems designed to react selectively with the thiol side chain of Cys in proteins are presented. Uniform attachment of a 21-amino acid peptide antigen through a single Cys residue with retention of biological function (antibody binding) has been attained. Further work with this system may lead to solutions for some of the problems which currently prevent the development of reliable biosensors for industrial and medical use.
ISSN:0169-4146
1476-5535
DOI:10.1007/BF01583997