Effects of vanadate on the ATP content, ATPase activity and phosphate absorption capacity of maize roots

Although the sensitivity of the plasma membrane H⁺-ATPase to vanadate is well known, the metabolic response of plant cells to vanadate is less well characterised in vivo and its use as an inhibitor in whole plant experiments has had mixed success. Experiments with maize (Zea mays, L.) roots and with...

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Bibliographic Details
Published in:Plant and soil 1994-11, Vol.167 (1), p.57-62
Main Authors: Sklenar, J. (Oxford Univ. (United Kingdom). Dept. of Plant Sciences), Fox, G.G, Loughman, B.C, Pannifer, A.D.B, Ratcliffe, R.G
Format: Article
Language:English
Subjects:
ABSORCION
ABSORPTION
Absorption. Translocation of ions and substances. Permeability
ADENOSINA TRIFOSFATASA
ADENOSINE TRIPHOSPHATASE
Adenosine triphosphatases
ADENOSINE TRIPHOSPHATE
ADENOSINTRIFOSFATO
Agronomy. Soil science and plant productions
Biological and medical sciences
Cell membranes
Cell physiology
Corn
Economic plant physiology
Enzymes
FOSFATOS
Fundamental and applied biological sciences. Psychology
Nutrition. Photosynthesis. Respiration. Metabolism
PHOSPHATE
PHOSPHATES
Plant physiology and development
Plant roots
Plant tissues
Plants
Plasma membrane and permeation
RACINE
RAICES
Root tips
ROOTS
Vanadates
VANADIO
VANADIUM
ZEA MAYS
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Summary:Although the sensitivity of the plasma membrane H⁺-ATPase to vanadate is well known, the metabolic response of plant cells to vanadate is less well characterised in vivo and its use as an inhibitor in whole plant experiments has had mixed success. Experiments with maize (Zea mays, L.) roots and with purified plasma membrane fractions from the same tissues showed that exposure to vanadate caused: (i) a reduction in the capacity for phosphate uptake; (ii) a reduction in the extractable ATPase activity from the tissue; and (iii) a significant increase in the ATP level. The measurements on the extractable ATPase activity and the ATP level showed that the effect of vanadate developed slowly, apparently reflecting the slow accumulation of intracellular vanadate. The marked effect of vanadate on the ATP level – exposure to 500 µM vanadate for 5 h doubled the ATP content of the roots tips – indicates that there is no stringent control over the ATP level in the roots and that the plasma membrane H⁺-ATPase activity is likely to have a significant role in determining the ATP level under normal conditions.
ISSN:0032-079X
1573-5036
DOI:10.1007/BF01587598