Morphological, immunocytochemical and growth characteristics of three human glioblastomas established in vitro

• Published in: Virchows Archiv A Pathological Anatomy and Histopathology 1991-07, Vol.418 (4), p.281-293
• Main Authors: BILZER, T, STAVROU, D, DAHME, E, KEIDITSCH, E, ANZIL, A. P, WECHSLER, W
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal - immunology; Antigens, Differentiation - metabolism; Antigens, Neoplasm - immunology; Antigens, Neoplasm - metabolism; Biological and medical sciences; Brain Neoplasms - genetics; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Brain Neoplasms - ultrastructure; CD57 Antigens; DNA, Neoplasm - analysis; DNA, Neoplasm - genetics; ErbB Receptors - metabolism; Female; Glial Fibrillary Acidic Protein - metabolism; Glioma - genetics; Glioma - metabolism; Glioma - pathology; Glioma - ultrastructure; Humans; Immunohistochemistry - methods; Male; Medical sciences; Microscopy, Electron; Microscopy, Electron, Scanning; Middle Aged; Neoplasm Transplantation; Ploidies; Receptors, Cell Surface - metabolism; Receptors, Nerve Growth Factor; Receptors, Transferrin - metabolism; S100 Proteins - metabolism; Tumor Cells, Cultured - metabolism; Tumor Cells, Cultured - pathology; Tumor Cells, Cultured - ultrastructure; Tumors
• ISSN: 0174-7398; 1432-2307
• DOI: 10.1007/bf01600156

The human glioblastoma-derived cell lines 86HG-39, 87HG-28 and 87HG-31, used for the production of monoclonal antibodies (mAbs) against glioma-associated antigens (GAA), were characterized in terms of morphology, growth behaviour, chromosomes and antigen expression. In the primary tumours, differential expression of glial fibrillary acidic protein, S100 protein, Leu-7 and GAA as defined by mAbs MUC 2-39, MUC 2-63 and MUC 8-22 was demonstrated. Receptors for epidermal growth factor (EGFr) and nerve growth factor (NGFr) were found in many cells in short-term cultures, but the transferrin receptor (Tr) was found in only a few cells of 87HG-28. In permanent cell lines, differentiation antigens and EGFr decreased and Tr increased markedly. NGFr and GAA remained stable. Transplantation tumours of 86HG-39 were partly positive for Tr and GAA. Chromosomal analysis revealed that the 86HG-39 and 87HG-28 cell lines had a hypodiploid or diploid stem line with lines in the hypotetraploid to tetraploid region for 50 in vitro passages. The 87HG-31 cell line had chromosomal patterns in the hypotriploid to triploid region. A gain of chromosomes was seen in the groups C7, C8, C10, D14, F19, F20, G21, G22. The variability of antigens in these tumours and especially during long-term cultivation probably reveals an ability to influence the growth of malignant glioma cells via the respective effector molecules.