Comparative immunohistochemical study of herpes simplex and varicella-zoster infections

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immuno...

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Bibliographic Details
Published in:Virchows Archiv A Pathological Anatomy and Histopathology 1993-03, Vol.422 (2), p.121-126
Main Authors: NIKKELS, A. F, DEBRUS, S, SADZOT-DELVAUX, C, PIETTE, J, DELVENNE, P, RENTIER, B, PIERARD, G. E
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antibodies, Monoclonal
Biological and medical sciences
Biopsy
Chickenpox - pathology
Dermatologie
Dermatology
Endothelium - chemistry
Endothelium - pathology
Herpes
Herpes Simplex - pathology
Herpesvirus 3, Human - isolation & purification
Human health sciences
Human viral diseases
Humans
Immunohistochemistry
Immunologie & maladie infectieuse
Immunology & infectious disease
Infectious diseases
Macrophages - chemistry
Macrophages - pathology
Medical sciences
Middle Aged
Sciences de la santé humaine
Simplexvirus - isolation & purification
Skin - chemistry
Skin - microbiology
Skin - pathology
Skin Diseases, Viral - microbiology
Skin Diseases, Viral - pathology
Skins
Varicella-zoster
Vasculitis
Viral diseases
Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
Viral Envelope Proteins - analysis
Viral Envelope Proteins - immunology
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Summary:Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387- and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis.
ISSN:0174-7398
1432-2307
DOI:10.1007/bf01607163