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Comparative immunohistochemical study of herpes simplex and varicella-zoster infections
Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immuno...
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| Published in: | Virchows Archiv A Pathological Anatomy and Histopathology 1993-03, Vol.422 (2), p.121-126 |
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| Main Authors: | , , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c419t-59250608e2d547f59c2d1a77340cebc090b185ef080fa5303c675e9d6f3566943 |
| container_end_page | 126 |
| container_issue | 2 |
| container_start_page | 121 |
| container_title | Virchows Archiv A Pathological Anatomy and Histopathology |
| container_volume | 422 |
| creator | NIKKELS, A. F DEBRUS, S SADZOT-DELVAUX, C PIETTE, J DELVENNE, P RENTIER, B PIERARD, G. E |
| description | Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387- and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis. |
| doi_str_mv | 10.1007/bf01607163 |
| format | article |
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F ; DEBRUS, S ; SADZOT-DELVAUX, C ; PIETTE, J ; DELVENNE, P ; RENTIER, B ; PIERARD, G. E</creator><creatorcontrib>NIKKELS, A. F ; DEBRUS, S ; SADZOT-DELVAUX, C ; PIETTE, J ; DELVENNE, P ; RENTIER, B ; PIERARD, G. E</creatorcontrib><description>Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387- and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis.</description><identifier>ISSN: 0174-7398</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/bf01607163</identifier><identifier>PMID: 8385379</identifier><identifier>CODEN: VAAHDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal ; Biological and medical sciences ; Biopsy ; Chickenpox - pathology ; Dermatologie ; Dermatology ; Endothelium - chemistry ; Endothelium - pathology ; Herpes ; Herpes Simplex - pathology ; Herpesvirus 3, Human - isolation & purification ; Human health sciences ; Human viral diseases ; Humans ; Immunohistochemistry ; Immunologie & maladie infectieuse ; Immunology & infectious disease ; Infectious diseases ; Macrophages - chemistry ; Macrophages - pathology ; Medical sciences ; Middle Aged ; Sciences de la santé humaine ; Simplexvirus - isolation & purification ; Skin - chemistry ; Skin - microbiology ; Skin - pathology ; Skin Diseases, Viral - microbiology ; Skin Diseases, Viral - pathology ; Skins ; Varicella-zoster ; Vasculitis ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye ; Viral Envelope Proteins - analysis ; Viral Envelope Proteins - immunology</subject><ispartof>Virchows Archiv A Pathological Anatomy and Histopathology, 1993-03, Vol.422 (2), p.121-126</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-59250608e2d547f59c2d1a77340cebc090b185ef080fa5303c675e9d6f3566943</citedby><cites>FETCH-LOGICAL-c419t-59250608e2d547f59c2d1a77340cebc090b185ef080fa5303c675e9d6f3566943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4657921$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8385379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NIKKELS, A. F</creatorcontrib><creatorcontrib>DEBRUS, S</creatorcontrib><creatorcontrib>SADZOT-DELVAUX, C</creatorcontrib><creatorcontrib>PIETTE, J</creatorcontrib><creatorcontrib>DELVENNE, P</creatorcontrib><creatorcontrib>RENTIER, B</creatorcontrib><creatorcontrib>PIERARD, G. E</creatorcontrib><title>Comparative immunohistochemical study of herpes simplex and varicella-zoster infections</title><title>Virchows Archiv A Pathological Anatomy and Histopathology</title><addtitle>Virchows Arch A Pathol Anat Histopathol</addtitle><description>Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387- and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Chickenpox - pathology</subject><subject>Dermatologie</subject><subject>Dermatology</subject><subject>Endothelium - chemistry</subject><subject>Endothelium - pathology</subject><subject>Herpes</subject><subject>Herpes Simplex - pathology</subject><subject>Herpesvirus 3, Human - isolation & purification</subject><subject>Human health sciences</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunologie & maladie infectieuse</subject><subject>Immunology & infectious disease</subject><subject>Infectious diseases</subject><subject>Macrophages - chemistry</subject><subject>Macrophages - pathology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Sciences de la santé humaine</subject><subject>Simplexvirus - isolation & purification</subject><subject>Skin - chemistry</subject><subject>Skin - microbiology</subject><subject>Skin - pathology</subject><subject>Skin Diseases, Viral - microbiology</subject><subject>Skin Diseases, Viral - pathology</subject><subject>Skins</subject><subject>Varicella-zoster</subject><subject>Vasculitis</subject><subject>Viral diseases</subject><subject>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><subject>Viral Envelope Proteins - analysis</subject><subject>Viral Envelope Proteins - immunology</subject><issn>0174-7398</issn><issn>1432-2307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNo9kM1v1DAQxa0K1G4Ll94r5YA4IIWOv-MjrGhBqsQFxNFynHHXVRIHO1lR_vqG7tLTjPR-72nmEXJJ4SMF0NdtAKpAU8VPyIYKzmrGQb8iG6Ba1Jqb5oycl_IAICVt5Ck5bXgjuTYb8mubhsllN8c9VnEYljHtYpmT3-EQveurMi_dY5VCtcM8YalKHKYe_1Ru7Kq9y9Fj37v6byoz5iqOAf0c01jekNfB9QXfHucF-Xnz5cf2a333_fbb9tNd7QU1cy0Nk6CgQdZJoYM0nnXUac0FeGw9GGjXgzFAA8FJDtwrLdF0KnCplBH8gtBDbh_xHm3KbbR7ZpOLh33p763ztkXLmGqs4rRZPe8Pnimn3wuW2Q6xPP8xYlqK1VIJAZKu4IcD6HMqJWOwU46Dy4-Wgv3XvP1887_5Fb46pi7tgN0Leqx61d8ddVfWYkN2o4_lBRNKasMofwL_HYre</recordid><startdate>199303</startdate><enddate>199303</enddate><creator>NIKKELS, A. 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| fulltext | fulltext |
| identifier | ISSN: 0174-7398 |
| ispartof | Virchows Archiv A Pathological Anatomy and Histopathology, 1993-03, Vol.422 (2), p.121-126 |
| issn | 0174-7398 1432-2307 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF01607163 |
| source | Springer LINK Archives |
| subjects | Adult Aged Aged, 80 and over Antibodies, Monoclonal Biological and medical sciences Biopsy Chickenpox - pathology Dermatologie Dermatology Endothelium - chemistry Endothelium - pathology Herpes Herpes Simplex - pathology Herpesvirus 3, Human - isolation & purification Human health sciences Human viral diseases Humans Immunohistochemistry Immunologie & maladie infectieuse Immunology & infectious disease Infectious diseases Macrophages - chemistry Macrophages - pathology Medical sciences Middle Aged Sciences de la santé humaine Simplexvirus - isolation & purification Skin - chemistry Skin - microbiology Skin - pathology Skin Diseases, Viral - microbiology Skin Diseases, Viral - pathology Skins Varicella-zoster Vasculitis Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Viral Envelope Proteins - analysis Viral Envelope Proteins - immunology |
| title | Comparative immunohistochemical study of herpes simplex and varicella-zoster infections |
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