Neutralization of inhibitory activity derived from cells of MDS patients and ML-2 cell line by monoclonal antibodies against H and L-subunit-rich ferritins

Monoclonal antibody 2A4 against human heart ferritin and a polyclonal antibody against human placental ferritin inactivated the suppressor activity present in media conditioned by cells of patients with myelodysplastic syndrome (MDS). These antibodies also neutralized the inhibitory activity release...

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Bibliographic Details
Published in:Blut 1990-11, Vol.61 (5), p.278-281
Main Authors: CUKROVA, V, NEUWIRTOVA, R
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Biological and medical sciences
Cell physiology
Culture Media
Ferritins - immunology
Fundamental and applied biological sciences. Psychology
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Humans
Immune Sera - immunology
Leukemia, Myeloid - metabolism
Leukemia, Myeloid - pathology
Macrophage Colony-Stimulating Factor - pharmacology
Molecular and cellular biology
Myelodysplastic Syndromes - metabolism
Myelodysplastic Syndromes - pathology
Responses to growth factors, tumor promotors, other factors
Tumor Cells, Cultured
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Summary:Monoclonal antibody 2A4 against human heart ferritin and a polyclonal antibody against human placental ferritin inactivated the suppressor activity present in media conditioned by cells of patients with myelodysplastic syndrome (MDS). These antibodies also neutralized the inhibitory activity released by cells of myeloid leukemic ML-2 cell line. In contrast, a monoclonal antibody against the ferritin L subunit did not neutralize the inhibitory activity of the media conditioned either by the cells of patients with MDS or by the ML-2 cell line. Neutralization of the inhibitory activity present in supernatants of cells of MDS patients and the ML-2 cell line by monoclonal antibodies against the H subunit of ferritin strongly indicates that the inhibitory activity resides in the acidic isoferritin molecule.
ISSN:0006-5242
1432-0584
DOI:10.1007/BF01732877