Assay and identification of histamine in human gastric aspirate by a fluorometric--fluoroenzymatic technique. Its application in patients with chronic duodenal ulcer

Histamine assays can be unreliable in individual subjects or samples even though the particular method is in general working very well. Therefore the specificity and accuracy of histamine determination in the gastric aspirate of individual duodenal ulcer patients was thoroughly examined and shown to...

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Bibliographic Details
Published in:Agents and Actions 1982-04, Vol.12 (1-2), p.17-25
Main Authors: Parkin, J V, Lorenz, W, Barth, H, Rohde, H, Ohmann, C, Thon, K, Weber, D, Crombach, M
Format: Article
Language:English
Subjects:
Adult
Animals
Duodenal Ulcer - metabolism
Gastric Juice - analysis
Histamine - analysis
Histamine N-Methyltransferase
Humans
Middle Aged
Pentagastrin
Spectrometry, Fluorescence - methods
Swine
Time Factors
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Summary:Histamine assays can be unreliable in individual subjects or samples even though the particular method is in general working very well. Therefore the specificity and accuracy of histamine determination in the gastric aspirate of individual duodenal ulcer patients was thoroughly examined and shown to be satisfactory. Pitfalls of the fluorometric assay were investigated. A native (non-histamine) fluorescence in gastric aspirate which occurs before the addition of OPT was not removed by the original Shore procedure. In the combined assay (Dowex 50 + butanol extraction) this fluorescence no longer interferes with the assay. For the identification of histamine in a single gastric aspirate of an individual duodenal ulcer patient, the reversed blank (3 M HCl added to the reaction mixture before OPT instead after OPT), excitation and fluorescence spectra, the heating test with spectra recorded and the HMT test were found to be reliable. The formaldehyde test and the heating test without recording the spectra were useless since they gave false negative results. Since the HMT test was regarded as a reference method it was thoroughly investigated both by theoretical considerations (enzyme kinetics) and by a series of measurements in a single patient as well as in a group of nine subjects. Samples from the period of peak acid output in response to pentagastrin showed an average histamine concentration of about 8 ng/ml and a histamine output of 1.5 microgram/30 min.
ISSN:0065-4299
1420-908X
DOI:10.1007/BF01965100