The phosphatidylinositol response of chondrocytes in culture

This study examined the phosphatidylinositol (PI) response in chondrocytes. The levels of 3H-inositol phosphates (IPs) were measured in guinea pig chondrocyte cultures labelled with 3H-myo-inositol and exposed to various inflammatory mediators and known agonists of the PI response, including bradyki...

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Bibliographic Details
Published in:Agents and Actions 1989-06, Vol.27 (3-4), p.428-430
Main Authors: Baragi, V M, Dudley, D T, Schrier, D J
Format: Article
Language:English
Subjects:
Animals
Bradykinin - pharmacology
Calcimycin - pharmacology
Carbachol - pharmacology
Cartilage, Articular - cytology
Cartilage, Articular - metabolism
Cells, Cultured
Guinea Pigs
Histamine - pharmacology
Phosphatidylinositols - biosynthesis
Stimulation, Chemical
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Summary:This study examined the phosphatidylinositol (PI) response in chondrocytes. The levels of 3H-inositol phosphates (IPs) were measured in guinea pig chondrocyte cultures labelled with 3H-myo-inositol and exposed to various inflammatory mediators and known agonists of the PI response, including bradykinin, carbachol, histamine, A23187, or recombinant human interleukin-1 beta. Of the mediators used to stimulate the PI response in chondrocytes, only bradykinin was effective. The bradykinin treated cells had higher levels of IPs (37-fold) than the controls. The PI response to bradykinin was dose and time dependent.
ISSN:0065-4299
1420-908X
DOI:10.1007/BF01972842