Some observations on the calcium ion binding to the eggshell matrix

The calcified matrix of the hen eggshell has been demineralized with the EDTA. Aliquots of this material are soluble in water and have been characterized by column chromatography and by chemical analyses. Of particular interest is the high hexosamine and uronic acid content, which confirms the prote...

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Bibliographic Details
Published in:Calcified Tissue Research 1978-12, Vol.26 (3), p.247-252
Main Authors: Abatangelo, G, Daga-Gordini, D, Castellani, I, Cortivo, R
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Animals
Binding Sites
Calcium
Carrier Proteins
Chemical Phenomena
Chemistry
Chickens
Egg Shell
Female
Glycoproteins
Hexosamines - analysis
Hydrogen-Ion Concentration
Kinetics
Uronic Acids - analysis
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Summary:The calcified matrix of the hen eggshell has been demineralized with the EDTA. Aliquots of this material are soluble in water and have been characterized by column chromatography and by chemical analyses. Of particular interest is the high hexosamine and uronic acid content, which confirms the protein-polysaccharide nature of this water-soluble material. The calcium ion binding to the eggshell matrix has been studied by the equilibrium dialysis technique at different pH values, with both free and blocked carboxylic groups. The material with the free carboxylic side chain groups binds more calcium ions with increasing pH value. When the carboxylic groups have been previously blocked with a water-soluble carbodiimide, the calcium ion binding rapidly decreases. The residual capacity to bind calcium ions in the material with the carboxylic functions modified is probably due to the sulfate ions. In agreement with previous observations on other calcified substrates, the calcium ion binding seems to depend on the presence of ionized carboxylic functions of the matrix.
ISSN:0008-0594
1432-0827
DOI:10.1007/bf02013266