Cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria

Monoclonal antibodies were produced against Escherichia coli O111, Escherichia coli J5, and the rough (R) mutant of Salmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identifie...

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Bibliographic Details
Published in:European Journal of Clinical Microbiology 1986-04, Vol.5 (2), p.148-151
Main Authors: de Jongh-Leuvenink, J, Bouter, A S, Marcelis, J H, Schellekens, J, Verhoef, J
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - analysis
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - immunology
Antibody Specificity
Antigens, Bacterial - immunology
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Escherichia coli - genetics
Escherichia coli - immunology
Immunosorbent Techniques
Lipopolysaccharides - immunology
Mice
Mice, Inbred BALB C
Mutation
O Antigens
Rabbits
Salmonella typhimurium
Salmonella typhimurium - genetics
Salmonella typhimurium - immunology
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Summary:Monoclonal antibodies were produced against Escherichia coli O111, Escherichia coli J5, and the rough (R) mutant of Salmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identified with an immunoblotting assay. Anti-Escherichia coli O111 monoclonal antibodies reacted only with homologous O antigens. Anti-J5 monoclonal antibodies cross-reacted with core lipopolysaccharide, especially with Rc lipopolysaccharide. IgM anti-J5 monoclonal antibodies showed more extensive cross-reactivity than IgG3 monoclonal antibodies. Anti-Re monoclonal antibodies cross-reacted weakly with all rough lipopolysaccharide tested. Thus, the varying specificity of these monoclonal antibodies seems to indicate that the core regions in the lipopolysaccharides of various gram-negative bacteria are not similar.
ISSN:0722-2211
1435-4373
DOI:10.1007/BF02013970