Immortalization of four new Fanconi anemia fibroblast cell lines by an improved procedure

Fanconi anemia (FA) is an autosomal recessive disease characterized by birth defects, progressive bone marrow failure and increased risk for leukemia. FA cells display chromosome breakage and increased cell killing in response to DNA crosslinking agents. At least 5 genes have been defined by cell co...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1996-03, Vol.22 (2), p.151-157
Main Authors: Jakobs, P M, Sahaayaruban, P, Saito, H, Reifsteck, C, Olson, S, Joenje, H, Moses, R E, Grompe, M
Format: Article
Language:English
Subjects:
Cell Culture Techniques
Cell Fusion
Cell Line, Transformed
Cell Survival - drug effects
Chromosome Aberrations
Epoxy Compounds - pharmacology
Ethyl Methanesulfonate - pharmacology
Fanconi Anemia
Fibroblasts - cytology
Fibroblasts - drug effects
Genetic Complementation Test
Humans
Karyotyping
Mitomycin - pharmacology
Mutagens - pharmacology
Phenotype
Skin - cytology
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Summary:Fanconi anemia (FA) is an autosomal recessive disease characterized by birth defects, progressive bone marrow failure and increased risk for leukemia. FA cells display chromosome breakage and increased cell killing in response to DNA crosslinking agents. At least 5 genes have been defined by cell complementation studies, but only one of these, FAC has been cloned to date. Efforts to map and isolate new FA genes by functional complementation have been hampered by the lack of immortalized FA fibroblast cell lines. Here we report the use of a novel immortalization strategy to create 4 new immortalized FA fibroblast lines, including one from the rare complementation group D.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF02369905