Detection and separation of the submetacentric marker chromosome of the WALKER (W-256) carcinoma using flow cytometry and sorting

The chromosomes of WALKER (W-256) carcinoma cells have been separated into different DNA subclasses using DAPI for quantitative DNA staining and laser flow cytometry. The submetacentric marker chromosome could be isolated and its DNA content was determined to be 1.3 pg. One microgram marker DNA was...

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Bibliographic Details
Published in:Histochemistry 1985-01, Vol.82 (5), p.469-475
Main Authors: HUTTER, K.-J, STOEHR, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinoma, Ehrlich Tumor - genetics
Cell Separation
Chromomycin A3
Chromosome Banding
Chromosomes - analysis
DNA - analysis
Female
Flow Cytometry
Genetic Markers
Host-tumor relations. Immunology. Biological markers
Indoles
Medical sciences
Neoplasm Transplantation
Rats
Rats, Inbred Strains
Reference Values
Tumors
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Summary:The chromosomes of WALKER (W-256) carcinoma cells have been separated into different DNA subclasses using DAPI for quantitative DNA staining and laser flow cytometry. The submetacentric marker chromosome could be isolated and its DNA content was determined to be 1.3 pg. One microgram marker DNA was obtained after separation of about 750 000 marker chromosomes by means of electronic flow sorting. The chromosomal composition of sorted fractions was analyzed by microscopy following banding of sorted chromosomes. The average morphological purity obtained was about 83%.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF02450482