DNA synthesis in lactotrophs of primary rat anterior pituitary cell cultures: effects of thyroliberin, bromocriptine and somatostatin

Prolactin immunostaining in combination with thymidine autoradiography was used to characterize changes in the DNA-synthesizing activity of lactotrophs in primary monolayer cultures of the rat anterior pituitary gland treated for 3 days with thyroliberin (TRH), somatostatin (SRIF) and bromocriptine...

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Bibliographic Details
Published in:Histochemistry 1985-01, Vol.82 (5), p.497-500
Main Authors: KOMOLOV, I. S, FEDOTOV, V. P, RAPPAY, G, FAZEKAS, I, GUDOSHNIKOV, V. I, BACSY, E
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Biological and medical sciences
Bromocriptine - pharmacology
Cells, Cultured
DNA - antagonists & inhibitors
DNA - biosynthesis
Female
Fundamental and applied biological sciences. Psychology
Hypothalamus. Hypophysis. Epiphysis. Urophysis
Immunoenzyme Techniques
Morphology. Functional localizations
Pituitary Gland, Anterior - cytology
Pituitary Gland, Anterior - metabolism
Prolactin - metabolism
Rats
Rats, Inbred Strains
Somatostatin - pharmacology
Thyrotropin-Releasing Hormone - pharmacology
Vertebrates: endocrinology
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Summary:Prolactin immunostaining in combination with thymidine autoradiography was used to characterize changes in the DNA-synthesizing activity of lactotrophs in primary monolayer cultures of the rat anterior pituitary gland treated for 3 days with thyroliberin (TRH), somatostatin (SRIF) and bromocriptine (CB 154). The number of lactotrophs labelled with 3H-thymidine within the total pool of labeled pituitary cells was used to estimate DNA synthesis in prolactin-producing cells. TRH (10 ng/ml) stimulated DNA synthesis in the whole population of cultured cells but not in lactotrophs. TRH only weakly counteracted the noticeable inhibitory effect of CB 154 (0.75 microM/l) on thymidine incorporation into lactotrophs. SRIF (20 ng/ml) inhibited DNA synthesis in lactotrophs to a lesser extent than CB 154. The combination of methods used in this paper may be useful for studying the selective effects of regulators on the proliferative activity of various pituitary cell types in vivo and in culture.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF02450486