Determination of monoamine transmitters and tyrosine in biological samples by capillary electrophoresis with electrochemical detection

Capillary electrophoresis (CE) has been employed for the separation of monoamine transmitters (MAs) and tyrosine (Tyr), combined with electrochemical detection (ED) at a carbon disc electrode. The effects of several factors such as the acidity and concentration of running buffer, the separation volt...

Full description

Saved in:
Bibliographic Details
Published in:Chromatographia 2000-08, Vol.52 (3-4), p.137-141
Main Authors: Chen, G, Ye, J. N, Cheng, J. S
Format: Article
Language:English
Subjects:
acidity
Analytical, structural and metabolic biochemistry
Biological and medical sciences
capillary electrophoresis
carbon
cerebral cortex
chromatography
detection limit
electrochemistry
electrodes
Fundamental and applied biological sciences. Psychology
Non peptidic neurotransmitters, polyamines
Other biological molecules
rats
spinal cord
thalamus
tyrosine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Capillary electrophoresis (CE) has been employed for the separation of monoamine transmitters (MAs) and tyrosine (Tyr), combined with electrochemical detection (ED) at a carbon disc electrode. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the potential applied to the working electrode and the injection time were investigated to find the optimum conditions. Detection limits (S/N=3) ranged from 48.8 to 315.4 nmol·L⁻¹, and the response was linear over 3 order of magnitude for MAs and Tyr. The proposed method was successfully applied to determine MAs and Tyr in the cerebral cortex, thalamus and spinal cord of rats with satisfactory assay results.
ISSN:0009-5893
1612-1112
DOI:10.1007/BF02490443