ESI-MS response characteristics of a synthetic peptide

ESI-MS response characteristics of a proprietary synthetic peptide are briefly studied through variation of solution composition, buffer content, liquid flow and nebulizer temperature. In accordance with Kebarle'sion competition theory, decreasing buffer concentration was seen to be well correl...

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Bibliographic Details
Published in:Chromatographia 2000, Vol.52 (S1), p.S60-S64
Main Authors: Zook, D. R, Forsmo-Bruce, H, Briem, S
Format: Article
Language:English
Subjects:
buffers
chromatography
electrospray ionization mass spectrometry
energy
ions
sodium
sodium chloride
surface tension
synthetic peptides
temperature
thermal energy
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Summary:ESI-MS response characteristics of a proprietary synthetic peptide are briefly studied through variation of solution composition, buffer content, liquid flow and nebulizer temperature. In accordance with Kebarle'sion competition theory, decreasing buffer concentration was seen to be well correlated to improved ESI-MS response. In line with general expectation, increased mobile phase organic content and heightened nebulizer temperature improved response, presumably factors promoting droplet fission/ion release via lowered droplet surface tension and added thermal energy, respectively. In contrast to expectation, added sodium chloride resulted in a modest signal increase despite concomitant formation of sodium adduct. A similar signal enhancement effect has been previously reported for quaternary ammonium halides, attributed to a thinning in the charged droplet electric bi-layer thereby promoting ion release.
ISSN:0009-5893
1612-1112
DOI:10.1007/bf02493125