Increased Cyclic Amp Content Directly Correlated with Morphological Transformation of Cells Infected with a Temperature-Sensitive Mutant of Mouse Sarcoma Virus

Normal rat kidney cells infected with a cold-sensitive mutant of mouse sarcoma virus [NRK(MSV-1b)] morphologically transform when exposed to adenosine 3':5' cyclic monophosphate (cAMP) at the restrictive temperature. The cAMP-induced morphological changes occur rapidly and are reversible....

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Bibliographic Details
Published in:In Vitro 1980-10, Vol.16 (10), p.851-858
Main Author: Somers, Kenneth D.
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Cell growth
Cell Line
Cell Transformation, Neoplastic
Cell Transformation, Viral
CHO cells
Cholera
Cholera Toxin - pharmacology
Cultured cells
Cyclic AMP - metabolism
Epidermal cells
Kidney cells
Mice
Mutation
Prostaglandins E - pharmacology
Rats
Sarcoma Viruses, Murine - physiology
Temperature
Toxins
Transformed cell line
Viral morphology
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Summary:Normal rat kidney cells infected with a cold-sensitive mutant of mouse sarcoma virus [NRK(MSV-1b)] morphologically transform when exposed to adenosine 3':5' cyclic monophosphate (cAMP) at the restrictive temperature. The cAMP-induced morphological changes occur rapidly and are reversible. Agents capable of elevating endogenous levels of cAMP [prostaglandin E1(PGE1) and cholera toxin (CT)] induced morphological transformation of NRK(MSV-1b) cells at the restrictive temperature that was concentration dependent, potentiated by cAMP phosphodiesterase inhibitors, and not prevented by inhibitors of DNA, RNA, and protein synthesis. Prostaglandin E1stimulated a transient increase in the intracellular level of cAMP with a concomitant morphological transformation and reversion of cells as cAMP levels decline. The maximum increase is reached by 10 min, followed by a decline to near basal level by 80 min. In contrast, incubation of cells with CT resulted in irreversible morphological transformation and increased levels of cAMP first detectable by 1 hr with maximum levels reached by 24 hr. Heated CT ($100\textdegree C$, 20 min) was without effect. Addition of CT to reverted$PGE_1-treated$cells resulted in morphological transformation suggesting the existence of discrete receptors in NRK(MSV-1b) cells.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02619422