Isozyme Differentiation of Aldolase and Pyruvate Kinase in Fetal, Regenerating, Preneoplastic, and Malignant Rat Hepatocytes during Culture

Aldolase and pyruvate kinase isozymes were investigated in cultured hepatocytes from fetal, regenerating, and 2-acetyl-aminofluorene-fed rat liver as well as in some epithelial liver cell lines. Our results show that: (a) cell proliferation and prolonged expression of specific isozymes were found on...

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Bibliographic Details
Published in:In Vitro 1981-05, Vol.17 (5), p.369-377
Main Authors: Guguen-Guillouzo, Christiane, Marie-France Szajnert, Glaise, Denise, Claudine Gregori, Fanny Schapira
Format: Article
Language:English
Subjects:
Animals
Cell culture techniques
Cell Line
Cell lines
Cells, Cultured
Cultured cells
Enzymes
Epithelial cells
Fructose-Bisphosphate Aldolase - metabolism
Hepatocellular carcinoma
Hepatocytes
Isoenzymes - metabolism
Liver
Liver - embryology
Liver - enzymology
Liver cells
Liver Neoplasms, Experimental - enzymology
Liver Regeneration
Pyruvate Kinase - metabolism
Rats
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Summary:Aldolase and pyruvate kinase isozymes were investigated in cultured hepatocytes from fetal, regenerating, and 2-acetyl-aminofluorene-fed rat liver as well as in some epithelial liver cell lines. Our results show that: (a) cell proliferation and prolonged expression of specific isozymes were found only in cultured hepatocytes from 17-day old fetuses; (b) the fetal type of pyruvate kinase expressed in regenerating and carcinogen-treated liver was temporarily lost only in cultured hepatocytes from regenerating liver; (c) the adult type of aldolase and pyruvate kinase was absent in one epithelial cell line derived from a carcinogen-treated liver and in the hepatoma tissue cell (HTC) line but was found in the Faza clone of the Reuber$H_{35}$cell line during the 50 first passages in vitro; and (d) the isozyme pattern of pyruvate kinase was always more strongly shifted than that of aldolase. The observations suggest that: (a) hepatocytes from carcinogen-treated liver exhibit the same lack of ability to proliferate in primary culture as normal adult hepatocytes; (b) adult hepatocytes can produce fetal isozymes without prior cell division; (c) pyruvate kinase is a stronger marker of dedifferentiation (retrodifferentiation) than aldolase; and (d) regulatory processes of isozyme expression are different during ontogenesis, regeneration, and hepatocarcinogenesis.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02626734