Characterization of cell-wall polymers from cotton ovule culture fiber cells by gel permeation chromatography

Cotton (Gossypium hirsutum, Texas Marker-1) fiber cells originating from ovule culture have been analyzed by gel permeation chromatography of dimethyl acetamide/lithium chloride-soluble components and compared with in planta-grown fibers. The profile of cell-wall polymer molecular weights indicated...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1995-07, Vol.31 (3), p.171-175
Main Authors: Triplett, B.A. (USDA, ARS, Southern Regional Research Center, New Orleans, LA.), Timpa, J.D
Format: Article
Language:English
Subjects:
CELL WALLS
CELLULOSE
CELLULOSES
CELULOSA
CHROMATOGRAPHIE
CHROMATOGRAPHY
Cotton
Cotton fibers
CROMATOGRAFIA
CULTIVO DE OVULOS
CULTURE D'OVULE
Cultured cells
Fiber cells
FIBRAS VEGETALES
FIBRE VEGETALE
FUZZ
Gel chromatography
GOSSYPIUM HIRSUTUM
Molecular weight
Molecules
OVULE CULTURE
Ovules
PARED CELULAR
PAROI CELLULAIRE
PELOS VEGETALES
Physiology
PLANT FIBRES
PLANT HAIRS
POIL VEGETAL
POLIMEROS
POLYMERE
POLYMERS
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Summary:Cotton (Gossypium hirsutum, Texas Marker-1) fiber cells originating from ovule culture have been analyzed by gel permeation chromatography of dimethyl acetamide/lithium chloride-soluble components and compared with in planta-grown fibers. The profile of cell-wall polymer molecular weights indicated that fibers grown for 21 d in culture more closely resembled fibers growing in planta for 30 d post-anthesis than fully mature fibers. The weight average molecular weight was 3 400 000 and number average molecular weight of polymers from ovule culture fibers was 109 000. Analysis of the polymer weight fraction distribution revealed that ovule culture fibers were similar to 30 d post-anthesis immature fibers but lacked a low molecular weight (log M 3-4) polymer fraction. Assessment of the polymer branching frequency showed that ovule culture fibers were intermediate in branching between 30 d post-anthesis fiber and mature in planta fiber. In summary, polymers deposited in cell walls of ovule culture fibers appear to grossly mimic the polymers accumulated during normal fiber biogenesis in planta, yet subtle differences may explain why ovule culture fibers rarely reach their full genetic potential in length.
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02632015