Glucose biosensor using glucose oxidase immobilized in polyaniline

A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5-4.0 min. In...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 1992-12, Vol.37 (3), p.267-273
Main Authors: PARENTE, A. H, MARQUES, E. T. A. JR, AZEVEDO, W. M, DINIZ, F. B, MELO, E. H. M, FILHO, J. L. L
Format: Article
Language:English
Subjects:
Aniline Compounds
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Buffers
Enzymes, Immobilized
Fundamental and applied biological sciences. Psychology
Glucose - analysis
Glucose Oxidase - metabolism
Glutaral
Methods. Procedures. Technologies
Polymers
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5-4.0 min. In addition, the immobilized enzyme had a higher activity between pH 6.5 and 7.5. The system retained 50% of its activity after 30 d of daily use. The optical absorption spectra of the polyaniline/glucose oxidase electrode after glucose had been added to the buffer solution showed that the absorption band around 800 nm had changed considerably when glucose was allowed to react with the electrode. This optical variation makes polyaniline a very promising polymer for use as a support in optical sensor for clinical application.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02788878