Primary Culture and Maintenance of Steroid-Secreting Human Placental Monolayers

A simple method is described for primary culture and for maintenance of hormone-producing cells from normal human placenta. A consistent yield of cells was obtained and an average survival of 3 to 4 months in culture using 1 mm³ explants from the most vascular area of the placentas. These explants w...

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Bibliographic Details
Published in:In Vitro 1976-02, Vol.12 (2), p.115-119
Main Authors: Roy, Pamela W., Ryan, Gail E., Bransome, Edwin D.
Format: Article
Language:English
Subjects:
Cell growth
Cell lines
Culture media
Cultured cells
Flasks
Horses
L cells
Placenta
Steroidogenesis
Steroids
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Summary:A simple method is described for primary culture and for maintenance of hormone-producing cells from normal human placenta. A consistent yield of cells was obtained and an average survival of 3 to 4 months in culture using 1 mm³ explants from the most vascular area of the placentas. These explants were placed in a variety of culture media in 30 ml flasks and incubated at 37°C in an atmosphere of 5% CO₂ and 95% air. The best yields in terms of cell growth were observed with Eagle's MEM (minimum essential medium) with supplements of horse serum and fetal calf serum or human cord serum. (Ham's F-10 with supplement of horse serum and fetal calf serum supports growth for the longest period and media containing human cord serum had the best yield of steroids.)
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02796357