Modified Nutrient Medium MCDB 151, Defined Growth Factors, Cholera Toxin, Pituitary Factors, and Horse Serum Support Epithelial Cell and Suppress Fibroblast Proliferation in Primary Cultures of Rat Ventral Prostate Cells

Nutrient medium WAJC 401 containing 5 μg/ml insulin, 10 ng/ml EGF, 10 ng/ml cholera toxin, 50 μg/ml pituitary extract, 1 μg/ml prolactin, 1 μM dexamethasone, and 5% horse serum supports the rapid proliferation of rat ventral prostate epithelial cells in primary culture. The same medium suppresses th...

Full description

Saved in:
Bibliographic Details
Published in:In Vitro 1982-02, Vol.18 (2), p.87-91
Main Authors: McKeehan, Wallace L., Adams, Pamela S., Rosser, Mary P.
Format: Article
Language:English
Subjects:
Animals
B lymphocytes
Blood
Cell aggregates
Cell Division - drug effects
Cell growth
Cells, Cultured
Cholera
Cholera Toxin - pharmacology
Culture Media
Cultured cells
Epidermal Growth Factor - pharmacology
Epithelial cells
Epithelium - drug effects
Epithelium - physiology
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - physiology
Growth Substances - pharmacology
Horses
Insulin - pharmacology
Male
Pituitary Gland - physiology
Prostate
Prostate - drug effects
Prostate - physiology
Rapid Communication
Rats
Tissue culture techniques
Tissue Extracts - pharmacology
Toxins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nutrient medium WAJC 401 containing 5 μg/ml insulin, 10 ng/ml EGF, 10 ng/ml cholera toxin, 50 μg/ml pituitary extract, 1 μg/ml prolactin, 1 μM dexamethasone, and 5% horse serum supports the rapid proliferation of rat ventral prostate epithelial cells in primary culture. The same medium suppresses the growth of prostate fibroblasts.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02796399