Micropropagation of 21 species of Mexican cacti by axillary proliferation

• Published in: In vitro cellular & developmental biology. Plant 1998-04, Vol.34 (2), p.131-135
• Main Authors: Molphe Balch, E.P, Perez Reyes, M.E, Villalobos Amador, E, Meza Rangel, E, Morones Ruiz, L. del R, Lizalde Viramontes, H.J
• Format: Article
• Language: English
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; Agronomy. Soil science and plant productions; AIA; AIB; ANA; APTITUD REGENERATIVA; APTITUDE A LA REGENERATION; BIOCHEMISTRY; BIOCHIMIE; Biological and medical sciences; BIOLOGICAL DIFFERENCES; BIOQUIMICA; Biotechnology; BOURGEON; BUDS; CACTACEAE; Cactus; CAL; CALLO; CALLUS; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CULTURE MEDIA; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; ENRACINEMENT; ENRAIZAMIENTO; Establishment of new cell lines, improvement of cultural methods, mass culture; Eukaryotic cell cultures; EXPLANT; EXPLANTES; EXPLANTS; Fundamental and applied biological sciences. Psychology; Genera; General agronomy. Plant production; Greenhouses; IAA; IBA; MEDIO DE CULTIVO; METHODE; METHODS; Methods. Procedures. Technologies; METODOS; MEXICO; MEXIQUE; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; NAA; Pectinatus; Plant cells and fungal cells; Plant tissues; Plants; PLANTULAS; PLANTULE; REGENERATIVE ABILITY; REPIQUAGE; ROOTING; SEEDLINGS; SPECIES DIFFERENCES; STEMS; SUPERVIVENCIA; SURVIE; SURVIVAL; TALLO; TIGE; TISSUE CULTURE; TRANSPLANTING; TRASPLANTE; Vegetative propagation. Micropropagation; Vegetative propagation. Sowing and planting. Harvesting; YEMA (PLANTA)
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/BF02822777

We have developed micropropagation systems for 21 species of Mexican cacti using explants from seedlings germinated in vitro or shoot segments of juvenile 2-3-yr-old greenhouse plants. The species propagated belong to the genera Astrophytum, Cephalocereus, Coryphantha, Echinocactus, Echinocereus, Echinofossulocactus, Ferocactus, Mammillaria, Nyctocereus, and Stenocactus. Multiple shoot formation from areoles was achieved in Murashige and Skoog (MS) medium supplemented with either 1 or 2 mg N⁶-benzyladenine (BA) per 1 (4.44 or 8.87 μM) or BA at 1 or 2 mg/l plus naphthaleneacetic acid at 0.1 or 1 mg/l (0.54 or 5.37 μM). The requirements of growth regulators for optimal shoot proliferation, the velocity of the response, and the number of buds produced by explant were different among the genera and species studied. Rooting of the shoots generated in vitro was achieved in MS medium supplemented with indoleacetic acid at 0.5-1 mg/l (2.85-5.71 μM) or indolebutyric acid at 0.5-1 mg/l (2.46-4.90 μM). Finally, 70-95% of the rooted plants transferred to potting medium survived.