Effects of Fe and Mn on the growth of a red tide dinoflagellateScrippsiella trochoidea (Stein) Loeblch III

• Published in: Chinese journal of oceanology and limnology 1997-06, Vol.15 (2), p.173-180
• Main Authors: Xiao-ming, Qin, Jing-zhong, Zhou, Pei-yuan, Qian
• Format: Article
• Language: English
• Subjects: Algae; Availability; Batch culture; Cell culture; Cell density; Cellular biology; Density; Ethylenediaminetetraacetic acids; Growth rate; Heavy metals; Iron; Manganese; Manganese ions; Marine biology; Photosynthesis; Physical growth; Red tide; Red tides; Saturation; Trace metals
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/BF02850689

Batch culture experiments were conducted with a red tide dinoflagellateScrippsiella trochoidea (Stein) Loeblch III collected from Jiaozhou Bay, Shangdong, China. Growth rates and oellular Chl--a were measured in media with iron and manganese ion concentrations controlled at different levels using EdTA-trace metal buffer systems. Cell density increased 3.2 times to 6.5 times over the range of lowest (0 mol/L) to highest (10^sup -5^ mol/L) iron and manganese ion concentrations. The range of cell density response was much lower than the range of total available iron and manganese, which was >100--fold that of Fe. This nonlinear relationship indicates that the cells adapt to make more efficient use of iron and manganese under limiting conditions. The cellular Chl--a content maximized after 3 days incubation and then decreased gradually under either iron or manganese limitation conditions. It indicated that the algae gained higher photosynthesis ability when transferred to a new environment. Growth responses to iron and manganese limitation can be both modeled according to the equation of Monod. The half--saturation constant for growth,k, is 4.6×10^sup -8^ mol/L for Fe and 5.1×10^sup -8^ mol/L for Mn. Our results showed that the iron availability in Jiaozhou Bay does not limit the growth ofS. trochoidea.[PUBLICATION ABSTRACT]