Expression of cytochrome P4502E1 in human liver: Relationship between genotype and phenotype in Chinese

Polymorphic cytochrome P4502E1 (CYP2E1) plays an important role in the metabolic activation of many carcinogens. We have previously shown that the c1/c1 genotype recognized by Rsa I in the 5'-regulatory region of the CYP2E1 may be a susceptibility factor for developing esophageal cancer and lun...

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Bibliographic Details
Published in:Science China. Life sciences 2001-08, Vol.44 (4), p.356-364
Main Authors: Tan, W, Wu, J, Tang, H, Lin, D
Format: Article
Language:English
Subjects:
Carcinogens
CYP2E1 protein
Cytochrome
Genotype & phenotype
Genotypes
Liver
Lung cancer
Metabolic activation
Metabolic rate
p-Nitrophenol
Phenotypes
Protein folding
Proteins
Susceptibility
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Summary:Polymorphic cytochrome P4502E1 (CYP2E1) plays an important role in the metabolic activation of many carcinogens. We have previously shown that the c1/c1 genotype recognized by Rsa I in the 5'-regulatory region of the CYP2E1 may be a susceptibility factor for developing esophageal cancer and lung cancer in Chinese. The present study was to investigate the relationship between the Rsa I genotype and the expression of CYP2E1 in human livers. A total of 50 liver specimens were genotyped for CYP2E1 and assayed for CYP2E1 protein contents and functional activity by using specific antibody in immunoblot and a probe substrate,p-nitrophenol. A considerable interindividual variation in CYP2E1 protein (20-fold) and functional activity (56-fold) was observed among these liver samples. However, when they were categorized according to genotype, the mean content of CYP2E1 protein was significantly higher among individuals with the c1/c1 genotype than that among those having c1/c2 or c2/c2 genotype [124.0+/-83.9 pmol/mg (n = 28) versus 65.5 +/-38.9 pmol/mg (n = 22),P
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/BF02879602