Preliminarily functional analysis of a cloned novel human geneADAM29

ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene—ADAM29, which was cloned in...

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Bibliographic Details
Published in:Science China. Life sciences 2001-08, Vol.44 (4), p.392-399
Main Authors: Wang, Fang, Xu, Rener, Zhu, Pengcheng, Hu, Junjie, Ying, Beibei, Zhao, Shouyuan, Li, Changben
Format: Article
Language:English
Subjects:
Fertilization
Inflammation
Interstitial cells
Membrane proteins
Metalloproteinase
Neurogenesis
Phylogeny
Signal transduction
Spermatogenesis
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Summary:ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene—ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA ofADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalianADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268—Asp374) as immunogen. The result of immunohistochemical test on human testis showed thatADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/BF02879606