Changes occurring in plasma membranes and intercellular junctions during the process of carcinogenesis and in squamous cell carcinoma

• Published in: Virchows Archiv. Abteilung B. Zellpathologie 1984-01, Vol.47 (1), p.1-15
• Main Authors: TACHIKAWA, T, YAMAMURA, T, YOSHIKI, S
• Format: Article
• Language: English
• Subjects: 9,10-Dimethyl-1,2-benzanthracene; Animals; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Carcinoma, Squamous Cell - etiology; Carcinoma, Squamous Cell - ultrastructure; Cell Membrane - ultrastructure; Cell Transformation, Neoplastic; Chemical agents; Freeze Fracturing; Intercellular Junctions - ultrastructure; Medical sciences; Microscopy, Electron; Mouth Mucosa; Mouth Neoplasms - etiology; Mouth Neoplasms - ultrastructure; Rats; Rats, Inbred Strains; Tumors
• ISSN: 0340-6075; 0042-6431
• DOI: 10.1007/BF02890184

A 0.5% mineral-oil solution of 9.10-dimethyl-1.2-benzanthracene (DMBA) was applied to artificial cecal pouches in the lower lips of rats. Ultrastructural studies were made of plasma membranes and intercellular junctions during the process of malignant transformation in the oral mucosal epithelium and after squamous cell carcinoma had been induced by the carcinogen. After the administration of DMBA, the inner leaflet of the membranes where the microfilaments are attached showed high electron density and intramembranous particles on the P-face of basal cells decreased to about half that of controls. However, on the E-face the number of intramembranous particles increased by approximately 10% compared with controls. Though the normal size range for intramembranous particles was 9-12 nm, the administration of DMBA caused aggregations of from three to six particles on the P-face. In squamous cell carcinomas, only the outer leaflet of the membranes showed high electron density; the number of intramembranous particles was 30% higher on the P-face and approximately three times higher on the E-face compared with controls and the morphology of the intramembranous particles, which formed irregular aggregates of from five to 20 particles, was specific. In animals treated with DMBA, the number of gap junctions decreased by between 50% and 70%, although no structural changes occurred. In squamous cell carcinomas, the area of gap junctions was about 50% lower and the number of gap junctions about 40% lower than in controls. Changes in the number and area of desmosomes were similar to those of gap junctions both in the DMBA-treated animals and in squamous cell carcinomas.