Genesis of unusual vesicles in rat periportal hepatocytes after administration of N-hydroxy-2-acetylaminofluorene

Administration of N-hydroxy-2-acetylaminofluorene (90 mumol/kg, iv) to rats results in damage to periportal hepatocytes. The most prominent ultrastructural changes are the appearance of numerous unusual vesicles of about 200 nm diameter and the proliferation of the endoplasmic reticulum to form fing...

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Bibliographic Details
Published in:Virchows Archiv. Abteilung B. Zellpathologie 1984, Vol.46 (1-2), p.77-82
Main Authors: GROOTHUIS, G. M. M, HULSTAERT, C. E, DHARAMDAJAL KALICHARAN, HARDONK, M. J
Format: Article
Language:English
Subjects:
2-Acetylaminofluorene - analogs & derivatives
5'-Nucleotidase
Adenosine Triphosphatases - metabolism
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Catalase - metabolism
Chemical agents
Endoplasmic Reticulum - drug effects
Endoplasmic Reticulum - ultrastructure
Glucose-6-Phosphatase - metabolism
Histocytochemistry
Hydroxyacetylaminofluorene - pharmacology
Liver - drug effects
Liver - enzymology
Liver - ultrastructure
Male
Medical sciences
Nucleotidases - metabolism
Rats
Rats, Inbred Strains
Tumors
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Summary:Administration of N-hydroxy-2-acetylaminofluorene (90 mumol/kg, iv) to rats results in damage to periportal hepatocytes. The most prominent ultrastructural changes are the appearance of numerous unusual vesicles of about 200 nm diameter and the proliferation of the endoplasmic reticulum to form fingerprint-like structures. In order to characterize these vesicles and to investigate their possible origin, their enzymatic activity was studied by ultrastructural enzyme histochemistry. The vesicles as well as the fingerprint-like structures exhibited glucose-6-phosphatase activity. Continuities between the vesicles and the membranes of both the RER and the SER were frequently seen. The vesicles lacked ATPase, 5'nucleotidase and catalase activity. From these results we conclude that the vesicles may be an unusual proliferation of the endoplasmic reticulum.
ISSN:0340-6075
0042-6431
DOI:10.1007/BF02890297