Reduction in striatal D2 dopamine receptor mRNA and binding following AF64A lesions

Unilateral lesions by a cholinotoxin, receptor autoradiography, and in situ hybridization techniques were employed to determine if dopaminergic receptors are located on cholinergic interneurons in the caudate-putamen (CPu). Lesion of the CPu with small amounts of the cholinotoxin AF64A resulted in a...

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Bibliographic Details
Published in:Molecular and chemical neuropathology 1993-09, Vol.20 (1), p.81-96
Main Authors: NEELAM NARANG, PUNDT, L. L, HUNT, M. E, ALBURGES, M. E, WAMSLEY, J. K
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Aziridines - toxicity
Base Sequence
Benzazepines - pharmacology
Biological and medical sciences
Caudate Nucleus - drug effects
Caudate Nucleus - metabolism
Choline - analogs & derivatives
Choline - toxicity
In Situ Hybridization
Medical sciences
Molecular Sequence Data
Nervous system (semeiology, syndromes)
Nervous system as a whole
Neurology
Neuromuscular Blocking Agents - toxicity
Neurons - drug effects
Neurons - metabolism
Putamen - drug effects
Putamen - metabolism
Rats
Receptors, Dopamine D1 - drug effects
Receptors, Dopamine D1 - metabolism
Receptors, Dopamine D2 - biosynthesis
Receptors, Dopamine D2 - drug effects
Receptors, Dopamine D2 - metabolism
RNA, Messenger - biosynthesis
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Summary:Unilateral lesions by a cholinotoxin, receptor autoradiography, and in situ hybridization techniques were employed to determine if dopaminergic receptors are located on cholinergic interneurons in the caudate-putamen (CPu). Lesion of the CPu with small amounts of the cholinotoxin AF64A resulted in a significant decrease in D2 receptor mRNA and D2 receptor binding. The loss was more pronounced in lateral and central portions of the CPu. Results obtained using [3H] SCH23390 binding to D1 receptors indicated that there was no change in this dopamine receptor subtype in the AF64A-lesioned CPu. A decrease in D2 receptor mRNA and receptor binding in AF64A-lesioned animals indicates that a population of postsynaptic D2 receptors is associated with the cholinergic interneurons. Lack of any change in [3H]SCH23390 binding in the AF64A-lesioned animals suggests that D1 receptors are not located on cholinergic neurons. These results provide evidence to support the selectivity of the lesion when used as indicated.
ISSN:1044-7393
2168-8729
DOI:10.1007/BF03160071