Differential gene expression profiling in B[k]F-exposed marine medaka,Oryzias javanicus by subtractive hybridization

Benzo[k]fluoranthene (B[k]F) is one of numerous polycyclic aromatic hydrocarbons (PAHs) which is a persistent environmental contaminant in air and water. Upon B[k]F exposure, differential gene expression profiling was conducted in marine medaka fish ( Oryzias javanicus ) using subtractive hybridizat...

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Bibliographic Details
Published in:Toxicology and environmental health sciences 2010, Vol.2 (4), p.231-237
Main Authors: Jeon, Hye-Young, Woo, Seonock, Won, Hyokyung, Kim, Bora, Yum, Seungshic
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Environmental Health
Pharmacology/Toxicology
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Summary:Benzo[k]fluoranthene (B[k]F) is one of numerous polycyclic aromatic hydrocarbons (PAHs) which is a persistent environmental contaminant in air and water. Upon B[k]F exposure, differential gene expression profiling was conducted in marine medaka fish ( Oryzias javanicus ) using subtractive hybridization. As a result, forty two differentially expressed candidate genes were induced in B[k]F-exposed fish as compared to control group, and the genes were associated with general metabolism, signal transduction, cell cycle, immune response, cytoskeleton, development, nucleotide/protein binding and some were non-categorized. These identified gene candidates have great potential to be developed as biomarkers for the identification of effects of B[k]F exposure in the environment. The results obtained in this study will offer insight to the physiological change induced by B[k]F exposure and will help assess the molecular mechanisms of B[k]F toxicity.
ISSN:2005-9752
DOI:10.1007/BF03217488