Biochemical and Cytological Studies During Seed Development in Somaclones of Lathyrus sativus

Soluble proteins in six somaclones and the parent at three different stages of development of Lathyrus sativus seeds showed polypeptides of mol wts ranging from 20–165 kD on SDS-PAGE. Immature seeds showed eight distinct esterase bands and two peroxidase bands. Pattern for both the enzymes among som...

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Bibliographic Details
Published in:Journal of plant biochemistry and biotechnology 1997-07, Vol.6 (2), p.101-104
Main Authors: Singh, Shalini, Johari, R. P, Mehta, S. L
Format: Article
Language:English
Subjects:
anaphase
chromosome number
chromosomes
developmental stages
Lathyrus sativus
metaphase
peroxidase
polyacrylamide gel electrophoresis
polypeptides
proteins
seed development
seeds
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Summary:Soluble proteins in six somaclones and the parent at three different stages of development of Lathyrus sativus seeds showed polypeptides of mol wts ranging from 20–165 kD on SDS-PAGE. Immature seeds showed eight distinct esterase bands and two peroxidase bands. Pattern for both the enzymes among somaclones was almost similar. During maturation a new isoform of peroxidase with high mobility appeared in all the somaclones, while one new isoform of intermediate mobility was visible in somaclones Bio L-08 and Bio I-08. Cytological analysis of all the somaclones confirmed the stability of the chromosome count of 2n=14. At metaphase I seven distinct bivalents and at anaphase I and II univalents showing complements of the seven chromosomes were visible. No structural or chromosomal aberration were observed among somaclones.
ISSN:0971-7811
0974-1275
DOI:10.1007/BF03263019