Molecular Characterisation of Low ODAP Somaclones of Lathyrus sativus

Somaclones of Lathyrus sativus having low ODAP contents were characterised at molecular level with respect to soluble protein pattern, esterase and ADH isozyme pattern, Southern hybridisation of genomic and mitochondrial DNA with specific probes. In 10-day-old seedling esterase isozyme pattern showe...

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Published in:Journal of plant biochemistry and biotechnology 1999, Vol.8 (1), p.25-29
Main Authors: Chakrabarti, Apratim, Santha, I. M, Mehta, S. L
Format: Article
Language:English
Subjects:
adenosinetriphosphatase
complementary DNA
hybridization
isozymes
Lathyrus sativus
mitochondrial DNA
seedlings
Southern blotting
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description Somaclones of Lathyrus sativus having low ODAP contents were characterised at molecular level with respect to soluble protein pattern, esterase and ADH isozyme pattern, Southern hybridisation of genomic and mitochondrial DNA with specific probes. In 10-day-old seedling esterase isozyme pattern showed the presence of an unique band of Rm 0.73 in Bio L-12 and 15-8-1 and the absence of a band of Rm 0.80 in 15-8-1, as compared to parent and other somaclones. Southern hybridisation of genomic DNA with cDNA clone 29 showed an extra band of 13.5 kb in L-56 and 15-8-1 only. Hybridisation of mitochondrial DNA with mitochondrial specific ati ATPase probe showed differences with the pattern from Bio R-15 being unique. Some of these differences observed will be useful as marker for their identification.
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subjects adenosinetriphosphatase
complementary DNA
hybridization
isozymes
Lathyrus sativus
mitochondrial DNA
seedlings
Southern blotting
title Molecular Characterisation of Low ODAP Somaclones of Lathyrus sativus
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