Isolation and characterization of a novel s-adenosyl-l-methionine synthetase cDNA from rice (Oryza satival l cv Pusa Basmati 1)

• Published in: Journal of plant biochemistry and biotechnology 2001-01, Vol.10 (1), p.25-29
• Main Authors: Mukhopadhyay, A, Sharma, S.(University of Delhi South Campus, New Delhi (India). Dept. of Plant Molecular Biology), Tyagi, A.K.(Chaudhary Charan Singh University, Meerut (India). Dept of Biotechnology)
• Format: Article
• Language: English
• Subjects: abscisic acid; ADN; cold stress; complementary DNA; DNA; drought; ESTRES; EXPRESION GENICA; expressed sequence tags; EXPRESSION DES GENES; GENE EXPRESSION; gene expression regulation; genes; GENOMAS; GENOME; GENOMES; LIGASAS; LIGASE; LIGASES; methionine adenosyltransferase; NUCLEOTIDE SEQUENCE; ORYZA SATIVA; ribonucleases; rice; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; STRESS
• ISSN: 0971-7811; 0974-1275
• DOI: 10.1007/BF03263102

An expression assay of randomly selected cDNAs resulted in the isolation of a novel cDNA (OSISAM) of SAM synthetase from rice. Nucleotide sequence data show that the cDNA is similar to a genomic clone (pRSAM1) from rice in the coding region but differs in the 5' untranslated region (UTR) that is unique to this cDNA. The RNase protection experiments and search of EST database confirmed the presence of such novel cDNA. Northern analysis shows that the gene is differentially expressed in various tissues. The transcript abundance also increases when plants are stressed with salt, drought, ABA, injury, osmotic- or heat-shock, but it is not influenced by cold stress.