RAPD analysis of indian isolates of rice sheath blight fungus Rhizoctonia solani

• Published in: Journal of plant biochemistry and biotechnology 2002-01, Vol.11 (1), p.43-48
• Main Authors: Neeraja, C.N, Vijayabhanu, N, Shenoy, V.V, Reddy, C.S, Sarma, N.P.(Directorate of Rice Research, Hyderabad (India))
• Format: Article
• Language: English
• Subjects: GENETIC VARIATION; ORYZA SATIVA; PATHOGENICITY; PATOGENICIDAD; POUVOIR PATHOGÈNE; RAPD; RHIZOCTONIA SOLANI; VARIACIÓN GENÉTICA; VARIATION GÉNÉTIQUE
• ISSN: 0971-7811; 0974-1275
• DOI: 10.1007/BF03263133

Rice sheath blight fungus Rhizoctonia solan! has a wide host range and is highly variable in pathogenecity, sclerotial production and cultural characteristics. In India, breeding for sheath blight resistant cultivars has been a priority area of research. However, lack of adequate information about the genetic variability of the fungal populations occurring in India, non-availability of appropriate markers and the non-availability of resistant donors are some of the limiting factors to achieve this objective. To assess the genetic variability in sheath blight fungus, 18 isolates collected from different rice growing regions of India were analyzed by using random amplified polymorphic DNA (RAPD) markers.The similarity values of RAPD profiles ranged from 0.41 to 0.85 with an average of 0.66 among all the isolates. The percentage polymorphism detected per primer varied from 79.2 to 100%. All the primers could be used to fingerprint the individual isolates. The cluster analysis using unweighted paired group method with arithmetic averages could distinguish between R. solani isolates as well as the virulent and avirulent isolates on rice.