Transgenic indica rice variety pusa basmati 1 constitutively expressing a rice chitinase gene exhibits enhanced resistance to rhizoctonia solani

Agrobacterium-mediated transformation of an elite indica rice variety, Pusa Basmati 1, was performed using LBA4404 (pSB1, pMKU-RF2) that harbours a rice chitinase gene (chi11) under the control of the maize ubiquitin (Ubi1) promoter-intron. Right border (gus) and left border (hph) flanking sequences...

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Bibliographic Details
Published in:Journal of plant biochemistry and biotechnology 2003-07, Vol.12 (2), p.93-101
Main Authors: Sridevi, G, Sabapathi, N, Meena, P, Veluthambi, K. (Madurai Kamaraj University, School of Biotechnology, Madurai (India). Department of Plant Biotechnology), Nandakumar, R, Samiyappan, R. (Tamil Nadu Agricultural University, Coimbatore (India). Department of Plant Pathology), Muthukrishnan, S. (Kansas State University, Manhattan (USA). Department of Biochemistry)
Format: Article
Language:English
Subjects:
agrobacterium
antifungal properties
blight
callus
chitinase
disease resistance
dna
electrophoresis
genetic engineering
homozygotes
immunoblotting
pathogenicity
rhizoctonia solani
rice
rna
segregation
transgenic plants
varieties
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Summary:Agrobacterium-mediated transformation of an elite indica rice variety, Pusa Basmati 1, was performed using LBA4404 (pSB1, pMKU-RF2) that harbours a rice chitinase gene (chi11) under the control of the maize ubiquitin (Ubi1) promoter-intron. Right border (gus) and left border (hph) flanking sequences and the transgene (chi11) in the middle of the T-DNA were used as probes in Southern analysis. Out of eleven independent To plants regenerated, three had single copy T-DNA insertions and eight had multiple T-DNA insertions. Nine To plants carried the complete T-DNA with the chitinase transgene. Two To plants did not carry chi11, though they had other T-DNA portions. Three plants harbouring single copy insertions and one plant harbouring two inserted copies were analyzed in detail. A segregation ratio of 3:1, reflecting T-DNA insertion at a single locus, was observed in the progeny of all the four To plants. Northern and western blot analyses of T1 plants revealed constitutive expression of chitinase at high levels. Bioassays of T1 plants indicated enhanced resistance to the sheath blight pathogen, Rhizoctonia solani, in comparison to control plants. Ahomozygous transgenic line was established from one To line, which exhibited the maximum resistance to R. Solani.
ISSN:0971-7811
0974-1275
DOI:10.1007/bf03263168