RNA-free DNA Extraction Protocol from PinusTissues for Molecular Biology or HPCE/HPLC Analyses

A simple method for extracting DNA from various in vitro or ex vitro Pinus tissues is described. Good yield and high quality RNA-free DNA ready to use in molecular biology assays or analytical analysis was obtained. DNA quality was measured analyzing absorption spectra between 200 and 300 nm, A₂₆₀/₂...

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Bibliographic Details
Published in:Journal of plant biochemistry and biotechnology 2009, Vol.18 (2), p.229-232
Main Authors: Valledor, L, Hasbún, R, Rodríguez, R, Cañal, M. J
Format: Article
Language:English
Subjects:
absorption
Biomedical and Life Sciences
callus
Cell Biology
DNA
high performance liquid chromatography
Life Sciences
molecular biology
Pinus
Plant Biochemistry
Protein Science
Receptors
scions
Short Communication
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Summary:A simple method for extracting DNA from various in vitro or ex vitro Pinus tissues is described. Good yield and high quality RNA-free DNA ready to use in molecular biology assays or analytical analysis was obtained. DNA quality was measured analyzing absorption spectra between 200 and 300 nm, A₂₆₀/₂₈₀ ratio and HPCE. This protocol was tested with no modification in a wide range of Pinus tissues from recalcitrant in vitro callus to mature field scions improving the results obtained with previous protocols. As the protocol is simple, almost universal and inexpensive it may be used for routine isolation from Pinus tissues. An isolation troubleshooting table was included to facilitate the setting up of reported protocol to other plant species.
ISSN:0971-7811
0974-1275
DOI:10.1007/BF03263325